产品货号：
GS2077
中文名称：
BstF5I内切酶
英文名称：
BstF5I
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.25.
技术规格
相关搜索：BstF5I内切酶，BstF5I限制性内切酶
使用说明
The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.25.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | GGATGNN↑ |
| 来源 | Bacillus stearothermophilus F5 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol;Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BseGI,BtsCI,FokI^ |
相关搜索：BstF5I内切酶，BstF5I限制性内切酶